Validating protein biomarkers november

The bands were directly cut out of the gels, destained with 50% acetonitrile in 25 m M ammonium bicarbonate and dried in a speed vacuum concentrator.Dried gel pieces were reswollen with 25 m M ammonium bicarbonate (p H 8.0) containing 50 ng trypsin and incubated at 37 °C for 16-24 h.When a regression model was applied, receiver operating characteristic analysis clearly discriminated MCD from the other causative diseases in NS.We developed a disease-specific protein panel that discriminated between three main causes of NS.In the discovery set, each individual sample was used for the patients with NS but pooled sample was analyzed for healthy controls.Sixty one urine samples from 51 patients with biopsy-proven 13 MCD, 26 MN, 5 FSGS, 9 Ig AN and 8 healthy controls were included in validation set (Table ).All the glomerulopathy was not secondary types caused by drugs, infection and malignancies, but primary disease.

Thus, there remains a need for new biomarkers to enable definitive diagnosis with an easy and non-invasive technique.Nephrotic syndrome (NS) is a nonspecific kidney disorder, commonly caused by minimal change disease (MCD), focal segmental glomerulosclerosis (FSGS), and membranous nephropathy (MN).Here we analyzed urinary protein profiles, aiming to discover disease-specific biomarkers of these three common diseases in NS.As a specimen, urine has several advantages compared with serum or plasma.Urine can be collected easily and non-invasively, and is free from other components that can interfere with blood analysis, such as clotting factors, active enzymes, and immunoregulatory proteins.